本剧集场景产生在佛罗里达州的迈阿密,以本地办案人员为故事主轴,描述他们是如何地透过高科技的帮助,以及巨细靡遗的传统办案伎俩,破解一个又一个待解的刑案。附属于迈阿密犯法鉴证科的犯法现场观察小组,频频收支种种案发明场寻找证据和蛛丝马迹,小组成员应用最先进的科技手腕,把外貌上没有任何意义的线索拼凑成不可反驳的证据,抽丝剥茧重组案情,如《犯法现场观察第三季》剧情那样让犯法分子无处可逃……CSI:CrimeSceneInvestigationtooktelevisionbystormin2000withitsinnovativeandcutting-edgeupdatingofastandardseriesconcept–inthiscase,thecopshow.Suddenly,insteadofsimplywhodunit,solvingcrimeswasamatterofhowdunit,andwithwhatsizeshoe,andwhetheranyoneleftbehindtracesofevidencethattheblueALSlightcouldpickup.NowthatgrittyapproachtocrimeknownasforensicsciencemakesitswayfromLasVegastothestreetsofFloridainCSI:Miami.AwholenewteamofcrimesceneinvestigatorsisonhandtoprobetheseamyundersideofwhatsomerefertoasCubaNorthandotherscallGeorgiaSouth.Consideritatropicalblendofpeople,culture,nightlifeand,ofcourse,theoccasionalkillingortwo.JustasyoudoinVegas,comeforthecrime,stickaroundfortheclues.Andthere"salwaysaclue.